BSL-3 Lab

The laboratory contains cutting edge equipment allowing advanced molecular-based and cell-based screens, as well as molecular and cell biology studies using live pathogens. In addition to standard equipment, these include an automated fluorescence microscope system (ScanR) equipped with a live imaging incubator, high NA objectives, filter wheels, online image analysis, a robotic station, an ultracentrifuge, a gradient maker and fractionator with inline UV and fluorescence cells, a plate reader, a NanoDrop, and additional equipment. See below for more details.  

 

 

olympus_scanning_microscope_ix

Olympus, Scanning Microscope IX-83

Automated imaging for accurate and efficient experiments designed for automated time lapse multidimensional and versatile fluorescence imaging and easy experiment setup. The system facilitates effortless data collection and seamless acquisition of panoramic, multichannel images.

Manufacturer Website

 

olympus_ix73_microscope

Olympus, IX73 Microscope

A microscope designed for efficient routine testing and other tasks such as observing cultures with DAPI, GFP and mCherry fluorescent signals

Manufacturer Website

liquid_handling_robot_tecan_freedom_evo

Liquid Handling Robot Tecan, Freedom Evo

Eight-channels liquid handling robot. Can be used for automated liquid preparation such as drug dilutions or cell cultures treatment.

Manufacturer Website

tecan_spark_microplate_reader

Tecan, Spark Microplate Reader

The Spark microplate reader is a multi-mode reader designed for detection for genomics, proteomics, microbiology, cell-based assays, and drug discovery. The reader measures absorbance in the 200-1000nm range, fluorescence, luminescence. It also has temperature-controlled chamber operating between 18-42C.

For complete Spark capabilities see Manufacturer Website 

beckman_coulter_optima_xpn-80_ultracentrifuge

Beckman Coulter, Optima XPN-80 Ultracentrifuge

Maximum speed of 80,000rpm (548,300xg) and maximum capacity of 1500 ml.

Manufacturer Website

Rotors available for this centrifuge

biocomp_gradient_forming_fractionating

BioComp, Gradient Forming & Fractionating

 

A newly developed hybrid instrument performs both gradient mixing (by the Gradient Master™) and fractionation (by the Piston Gradient Fractionator™). BioComp Gradient Former (e.g. sucrose, glycerol, optiprep etc) and Fractionator, comprises a UV and fluorescence flow cell and an automatic fraction collector.

 

Manufacturer Website

 

 

 

thermofisher_nanodrop_one

ThermoFisher, NanoDrop One

NanoDrop One measures absorbance in wide spectral range of 190-850nm and can be used for detection of:

  • Peptides (205 nm)
  • DNA and RNA (260 nm)
  • Purified protein (280 nm)
  • Toxicology assays and industrial dyes (490 nm)
  • Gold nanoparticles (520 nm)
  • Colorimetric protein assays (BCA 562 nm, Bradford 595 nm, Modified Lowry 650 nm, Pierce 660 660 nm)
  • Optical density measurements (600 nm)

For complete Spark capabilities see Manufacturer Website


The BSL-3 Unit is also equipped with basic instruments such as: refrigerated centrifuges, heating block with shaking, a scale, an oven, refrigerators, -20C and -80C freezers, incubators with CO2 and incubators with CO2 + O2 controls, biological safety cabinets and chemical safety cabinet for fixation, anesthesia and dissections. A double-sided autoclave is also stationed in the unit for decontamination and sterilization.

 

For questions and orders contact us


 Beckman Coulter, Optima XPN-80 Ultracentrifuge rotors: 

Rotor Type

Manufacturer

Max Speed (RPM)

Relative Centrifugal Field (x g) at rmax

Number of Positions

Max Tube Volume

Catalogue No of Tubes

Ti70-Fixed

Beckman

70K

504,000

8

39 mL

Ultra-Clear tubes 38.5mL – 344058

Polyallomer tubes 38.5mL - 326823

SW41 Ti-Swinging

Beckman

41K

288,000

6

13.2 mL

Ultra-Clear 344059 Polypropylene - 331372

SW32 Ti-Swinging

Beckman

32K

175,000

6

38.5 mL

Ultra-Clear tubes 38.5mL – 344058

Polyallomer tubes 38.5mL – 326823